Targeted Deletion of Hsf1, 2, and 4 Genes in Mice

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Scopus citations


Heat-shock transcription factors (Hsfs) regulate transcription of heat-shock proteins as well as other genes whose promoters contain heat-shock elements. There are at least five Hsfs in mammalian cells, Hsf1, Hsf2, Hsf3, Hsf4, and Hsfy. To understand the physiological roles of Hsf1, Hsf2, and Hsf4 in vivo, we generated knockout mouse lines for these factors. In this chapter, we describe the design of the targeting vectors, the plasmids used, and the successful generation of mice lacking the individual genes. We also briefly describe what we have learned about the physiological functions of these genes in vivo.

Original languageEnglish (US)
Title of host publicationMolecular Chaperones
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages20
ISBN (Print)9781617792946
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Hsf1
  • Hsf2
  • Hsf4
  • Hsf4-EGFP
  • Knockout mice
  • Targeting vector

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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