@inbook{5a0dcd09084a40379de02ee961fb3277,
title = "Targeted Deletion of Hsf1, 2, and 4 Genes in Mice",
abstract = "Heat-shock transcription factors (Hsfs) regulate transcription of heat-shock proteins as well as other genes whose promoters contain heat-shock elements. There are at least five Hsfs in mammalian cells, Hsf1, Hsf2, Hsf3, Hsf4, and Hsfy. To understand the physiological roles of Hsf1, Hsf2, and Hsf4 in vivo, we generated knockout mouse lines for these factors. In this chapter, we describe the design of the targeting vectors, the plasmids used, and the successful generation of mice lacking the individual genes. We also briefly describe what we have learned about the physiological functions of these genes in vivo.",
keywords = "Hsf1, Hsf2, Hsf4, Hsf4-EGFP, Knockout mice, Targeting vector",
author = "Xiongjie Jin and Binnur Eroglu and Demetrius Moskophidis and Mivechi, {Nahid F.}",
note = "Funding Information: This work was supported by VA Award 1I01BX000161 and NIH grants CA062130 and CA132640 (NFM) and CA121951 and CA121951-07S2 (DM). For generation of hsf knockout mice, the microinjection of ES cells and generation of chimeras were conducted in the Medical College of Georgia Embryonic Stem Cell and Transgenic Core Facility. Publisher Copyright: {\textcopyright} 2011, Springer Science+Business Media, LLC.",
year = "2011",
doi = "10.1007/978-1-61779-295-3_1",
language = "English (US)",
isbn = "9781617792946",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "1--20",
booktitle = "Molecular Chaperones",
}