Tensile strength and microhardness of treated human dentin

Victoria Fuentes, Laura Ceballos, Raquel Osorio, Manuel Toledano, Ricardo M. Carvalho, David H. Pashley

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


Objectives. To determine the ultimate tensile strength and Knoop hardness of mineralized, EDTA-treated, sodium hypochlorite (NaOCl)-treated, EDTA-treated resin-infiltrated, and NaOCl-treated resin-infiltrated dentin. Methods. Dumbell-shaped specimens with a cross-sectional area of 0.5 mm2 were prepared from the crowns of extracted human third molars. Specimens were randomly assigned to the following experimental groups: (1) mineralized dentin; (2) 0.5 M EDTA-demineralized dentin, pH 7/5 days; (3) 5% NaOCl-deproteinized dentin/2 days; (4) EDTA-treated, Single Bond resin-infiltrated dentin; (5) NaOCl-treated, Single Bond resin-infiltrated dentin. All specimens were tested in tension in a Vitrodyne testing machine at 0.6 mm/min. Knoop microhardness was measured on the fractured edges of specimens in groups 1, 3, 4, and 5. Results were analyzed by ANOVA and SNK tests (p<0.05). Results. Both EDTA and NaOCl treatments caused significant reductions in the tensile strength and microhardness of mineralized dentin (p<0.05), with the largest reductions observed after NaOCl treatment (p<0.05). Resin infiltration of treated dentin resulted in moderate increase of its tensile strength and microhardness, however, the original mineralized values were not recovered (p<0.05). Significance. Whenever dentin surfaces are treated with EDTA or NaOCl prior to a clinical bonding procedure, clinicians must be aware that a weak layer may be present at the interface, which may lead to premature failures of resin/dentin bonds.

Original languageEnglish (US)
Pages (from-to)522-529
Number of pages8
JournalDental Materials
Issue number6
StatePublished - Jul 2004


  • Dentin
  • Microhardness
  • Microtensile
  • Tensile strength

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials


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