TY - JOUR
T1 - Transforming growth factor-β1 modulates expression of adhesion and cytoskeletal proteins in human peritoneal fibroblasts
AU - Rout, Ujjwal K.
AU - Saed, Ghassan M.
AU - Diamond, Michael P.
N1 - Funding Information:
The authors thank the Confocal Microscopy Core facility sponsored by the Institute of Chemical Toxicology, Wayne State University (WSU), Detroit, Michigan, and the Image Analysis Facility of D.R. Armant, Ph.D. U.K. Rout, Ph.D., acknowledges a 1999–2000 WSU Department of Obstetrics and Gynecology Research Grant.
PY - 2002
Y1 - 2002
N2 - Objective: To determine the effects of TGF-β1 on the expression of the α1, α2, α5, αv, and α6 integrin subunits and on vinculin, and F-actin in human peritoneal fibroblasts. Design: Descriptive study using cell culture, reverse transcriptase-polymerase chain reaction (RT-PCR), and immunofluorescent and confocal microscopy. Setting: Academic medical center. Patient(s): Gynecological surgery patients. Intervention(s): None. Main Outcome Measure(s): Effects of TGF-β1 on the steady state levels of α5, αv, and α6 integrin transcripts were examined in the normal peritoneal fibroblasts using RT-PCR. Expression levels of the α1, α2, α5, αv, and α6 integrin subunits and of F-actin were measured by immunofluorescent microscopy. The distribution pattern of the integrin subunits, vinculin, and F-actin were examined using confocal microscopy. Result(s): TGF-β1 significantly up-regulated the expression levels of the α5, αv, and α6 integrin subunits and modulated their expression pattern, resulting in relatively higher levels of these subunits in the focal contacts of peritoneal fibroblasts. It allocated vinculin expression primarily to the focal contacts of cells and caused distortion of F-actin structure. The transcript levels of the α5, αv, and α6 integrin subunits were not altered by TGF-β1 treatment. Conclusion(s): TGF-β1 may promote postoperative adhesion formation by inducing the migration of peritoneal fibroblasts by altering the expression levels and patterns of specific integrin subunits, vinculin, and F-actin.
AB - Objective: To determine the effects of TGF-β1 on the expression of the α1, α2, α5, αv, and α6 integrin subunits and on vinculin, and F-actin in human peritoneal fibroblasts. Design: Descriptive study using cell culture, reverse transcriptase-polymerase chain reaction (RT-PCR), and immunofluorescent and confocal microscopy. Setting: Academic medical center. Patient(s): Gynecological surgery patients. Intervention(s): None. Main Outcome Measure(s): Effects of TGF-β1 on the steady state levels of α5, αv, and α6 integrin transcripts were examined in the normal peritoneal fibroblasts using RT-PCR. Expression levels of the α1, α2, α5, αv, and α6 integrin subunits and of F-actin were measured by immunofluorescent microscopy. The distribution pattern of the integrin subunits, vinculin, and F-actin were examined using confocal microscopy. Result(s): TGF-β1 significantly up-regulated the expression levels of the α5, αv, and α6 integrin subunits and modulated their expression pattern, resulting in relatively higher levels of these subunits in the focal contacts of peritoneal fibroblasts. It allocated vinculin expression primarily to the focal contacts of cells and caused distortion of F-actin structure. The transcript levels of the α5, αv, and α6 integrin subunits were not altered by TGF-β1 treatment. Conclusion(s): TGF-β1 may promote postoperative adhesion formation by inducing the migration of peritoneal fibroblasts by altering the expression levels and patterns of specific integrin subunits, vinculin, and F-actin.
KW - Adhesion
KW - F-actin
KW - Immunofluorescence
KW - Integrin
KW - PCR
KW - Peritoneal fibroblasts
KW - TGF-β
KW - Vinculin
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U2 - 10.1016/S0015-0282(02)03176-X
DO - 10.1016/S0015-0282(02)03176-X
M3 - Article
C2 - 12095506
AN - SCOPUS:0036293914
SN - 0015-0282
VL - 78
SP - 154
EP - 161
JO - Fertility and sterility
JF - Fertility and sterility
IS - 1
ER -