Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells

Wujun Zhao; Yang Liu; Brittany D. Jenkins; Rui Cheng; Bryana N. Harris; Weizhong Zhang; Jin Xie; Jonathan R Murrow; Jamie Hodgson; Mary Egan; Ana Bankey; Petros G. Nikolinakos; Haythem Y. Ali; Kristina Meichner; Lisa A. Newman; Melissa B Davis; Leidong Mao

doi:10.1039/c9lc00210c

Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells

Wujun Zhao, Yang Liu, Brittany D. Jenkins, Rui Cheng, Bryana N. Harris, Weizhong Zhang, Jin Xie, Jonathan R Murrow, Jamie Hodgson, Mary Egan, Ana Bankey, Petros G. Nikolinakos, Haythem Y. Ali, Kristina Meichner, Lisa A. Newman, Melissa B Davis, Leidong Mao

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Isolation of circulating tumor cells (CTCs) from blood provides a minimally-invasive alternative for basic understanding, diagnosis, and prognosis of metastatic cancer. The roles and clinical values of CTCs are under intensive investigation, yet most studies are limited by technical challenges in the comprehensive enrichment of intact and viable CTCs with minimal white blood cell (WBC) contamination. Here, we report a novel method based on contrast of cell magnetization in biocompatible ferrofluids (a colloidal magnetic nanoparticle suspension), termed as integrated ferrohydrodynamic cell separation (iFCS), that enriches CTCs in a tumor antigen-independent and cell size variation-inclusive manner, achieves a high throughput (12 mL h^-1), high recovery rate (99.08% at down to ∼10 cells per mL spike ratio), and low WBC contamination (533 cells for every one milliliter blood processed) and is biocompatible. This method will enable large cohort research to define the clinical and diagnostic value of CTC subtypes.

Original language	English (US)
Pages (from-to)	1860-1876
Number of pages	17
Journal	Lab on a Chip
Volume	19
Issue number	10
DOIs	https://doi.org/10.1039/c9lc00210c
State	Published - May 21 2019
Externally published	Yes

ASJC Scopus subject areas

Bioengineering
Biochemistry
General Chemistry
Biomedical Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1039/c9lc00210c

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Zhao, W., Liu, Y., Jenkins, B. D., Cheng, R., Harris, B. N., Zhang, W., Xie, J., Murrow, J. R., Hodgson, J., Egan, M., Bankey, A., Nikolinakos, P. G., Ali, H. Y., Meichner, K., Newman, L. A., Davis, M. B., & Mao, L. (2019). Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells. Lab on a Chip, 19(10), 1860-1876. https://doi.org/10.1039/c9lc00210c

Zhao, W, Liu, Y, Jenkins, BD, Cheng, R, Harris, BN, Zhang, W, Xie, J, Murrow, JR, Hodgson, J, Egan, M, Bankey, A, Nikolinakos, PG, Ali, HY, Meichner, K, Newman, LA, Davis, MB & Mao, L 2019, 'Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells', Lab on a Chip, vol. 19, no. 10, pp. 1860-1876. https://doi.org/10.1039/c9lc00210c

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abstract = "Isolation of circulating tumor cells (CTCs) from blood provides a minimally-invasive alternative for basic understanding, diagnosis, and prognosis of metastatic cancer. The roles and clinical values of CTCs are under intensive investigation, yet most studies are limited by technical challenges in the comprehensive enrichment of intact and viable CTCs with minimal white blood cell (WBC) contamination. Here, we report a novel method based on contrast of cell magnetization in biocompatible ferrofluids (a colloidal magnetic nanoparticle suspension), termed as integrated ferrohydrodynamic cell separation (iFCS), that enriches CTCs in a tumor antigen-independent and cell size variation-inclusive manner, achieves a high throughput (12 mL h-1), high recovery rate (99.08% at down to ∼10 cells per mL spike ratio), and low WBC contamination (533 cells for every one milliliter blood processed) and is biocompatible. This method will enable large cohort research to define the clinical and diagnostic value of CTC subtypes.",

author = "Wujun Zhao and Yang Liu and Jenkins, {Brittany D.} and Rui Cheng and Harris, {Bryana N.} and Weizhong Zhang and Jin Xie and Murrow, {Jonathan R} and Jamie Hodgson and Mary Egan and Ana Bankey and Nikolinakos, {Petros G.} and Ali, {Haythem Y.} and Kristina Meichner and Newman, {Lisa A.} and Davis, {Melissa B} and Leidong Mao",

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AU - Zhang, Weizhong

AU - Xie, Jin

AU - Murrow, Jonathan R

AU - Hodgson, Jamie

AU - Egan, Mary

AU - Bankey, Ana

AU - Nikolinakos, Petros G.

AU - Ali, Haythem Y.

AU - Meichner, Kristina

AU - Newman, Lisa A.

AU - Davis, Melissa B

AU - Mao, Leidong

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Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells

Abstract

ASJC Scopus subject areas

UN SDGs

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