Two-photon laser confocal microscopy of micropermeability of resin-dentin bonds made with water or ethanol wet bonding

Salvatore Sauro, Timothy F. Watson, Francesco Mannocci, Katsuya Miyake, Bradford P. Huffman, Franklin R. Tay, David H. Pashley

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


This study evaluated the micropermeability of six etch-and-rinse adhesives bonded to dentin. There were two principal groups: wet bonding with water or wet bonding with absolute ethyl alcohol. After bonding and the creation of composite build-ups, the pulp chambers were filled with 0.1% lucifer yellow. The contents of the pulp chamber were kept under 20 cm H2O pressure to simulate pulpal pressure for 3 h. The specimens were vertically sectioned into multiple 0.5-mm thick slabs that were polished and then examined using a twophoton confocal laser scanning microscope (TPCLSM). The results showed that specimens bonded with adhesives using the water wet-bonding condition all showed tracer taken up uniformly by the hybrid layer. This uptake of fluorescent tracer into the hybrid layer was quantified by computer software. The most hydrophobic experimental resins showed the highest fluorescent tracer uptake (ca. 1800 ± 160 arbitrary fluorescent units/std. surface area). The most hydrophilic experimental resins showed the lowest tracer uptake into watersaturated hybrid layers. When ethanol wet-bonding was used, significantly less fluorescent tracer was seen in hybrid layers. The most hydrophilic experimental resins and Single Bond Plus showed little micropermeability. Clearly, ethanol wet-bonding seals dentin significantly better than water-wet dentin regardless of the adhesive in etch-and-rinse systems.

Original languageEnglish (US)
Pages (from-to)327-337
Number of pages11
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume90 B
Issue number1
StatePublished - Jul 2009


  • Dentin sealing
  • Ethanol-saturated dentin
  • Hydrophobic hybrid layer
  • Micropermeability
  • Two-photon confocal microscopy

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering


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