TY - JOUR
T1 - Upregulation of the chemokine monocyte chemoattractant protein-1 following unilateral nerve injury in the peripheral taste system
AU - Cavallin, Melissa Ann
AU - McCluskey, Lynnette Phillips
N1 - Funding Information:
This work is supported by Grants NIH DC005811 (L.P.M.) and DC008263 (M.A.C.).
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007/2/21
Y1 - 2007/2/21
N2 - Macrophages are recruited to both sides of the tongue following unilateral chorda tympani (CT) nerve injury. The mechanisms responsible for recruiting these macrophages to the peripheral taste system are unknown. Neural degeneration in other systems leads to the upregulation of small molecules that function as chemoattractant cytokines, or chemokines. The chemokines monocyte chemoattractant protein (MCP)-1 and macrophage inflammatory protein (MIP)-1α are important regulators of macrophage recruitment to sites of infection and injury. We hypothesized that CT nerve sectioning leads to a bilateral upregulation of MCP-1 and MIP-1α. We examined lingual protein levels of MCP-1 and MIP-1α by enzyme-linked immunosorbent assays (ELISA)s at several time points after unilateral CT section in rats. MCP-1 was significantly upregulated on the intact side of the tongue at 12 h after sectioning, and on the injured side at 24-48 h post-injury. However, MIP-1α expression did not significantly change following CT nerve sectioning. These data indicate that chemokines are differentially regulated following neural injury, and that MCP-1 may contribute to the bilateral macrophage response to neural injury. Furthermore, the increase in MCP-1 occurs even in uninjured, distant sites, and may be upstream from the deficits in neural responses from the contralateral CT after sectioning.
AB - Macrophages are recruited to both sides of the tongue following unilateral chorda tympani (CT) nerve injury. The mechanisms responsible for recruiting these macrophages to the peripheral taste system are unknown. Neural degeneration in other systems leads to the upregulation of small molecules that function as chemoattractant cytokines, or chemokines. The chemokines monocyte chemoattractant protein (MCP)-1 and macrophage inflammatory protein (MIP)-1α are important regulators of macrophage recruitment to sites of infection and injury. We hypothesized that CT nerve sectioning leads to a bilateral upregulation of MCP-1 and MIP-1α. We examined lingual protein levels of MCP-1 and MIP-1α by enzyme-linked immunosorbent assays (ELISA)s at several time points after unilateral CT section in rats. MCP-1 was significantly upregulated on the intact side of the tongue at 12 h after sectioning, and on the injured side at 24-48 h post-injury. However, MIP-1α expression did not significantly change following CT nerve sectioning. These data indicate that chemokines are differentially regulated following neural injury, and that MCP-1 may contribute to the bilateral macrophage response to neural injury. Furthermore, the increase in MCP-1 occurs even in uninjured, distant sites, and may be upstream from the deficits in neural responses from the contralateral CT after sectioning.
KW - Chorda tympani nerve
KW - Dietary sodium restriction
KW - Macrophage recruitment
KW - Neural degeneration
KW - Neuroimmune interactions
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U2 - 10.1016/j.neulet.2006.11.042
DO - 10.1016/j.neulet.2006.11.042
M3 - Article
C2 - 17174471
AN - SCOPUS:33846785592
SN - 0304-3940
VL - 413
SP - 187
EP - 190
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 3
ER -