TY - JOUR
T1 - Use of a glycomics array to establish the anti-carbohydrate antibody repertoire in type 1 diabetes
AU - Tran, Paul M.H.
AU - Dong, Fran
AU - Kim, Eileen
AU - Richardson, Katherine P.
AU - Tran, Lynn K.H.
AU - Waugh, Kathleen
AU - Hopkins, Diane
AU - Cummings, Richard D.
AU - Wang, Peng George
AU - Rewers, Marian J.
AU - She, Jin Xiong
AU - Purohit, Sharad
N1 - Funding Information:
This work was supported by National Institute of Health (NIH)/ National Cancer Institute (NCI) grant 1 R21 CA199868 and U01CA221242 to J.X.S., P.G.W., R.D.C., and S.P., as well as the P41GM103694 and R24GM137763 to R.D.C. PMHT was supported by NIH/NIDDK fellowship (F30DK121461). Diabetes Autoimmunity Study in the Young (M.R., K.W., and F.D.) is supported by the National Institutes of Health (R01 DK032493 and P30 DK116073) and Helmsley Charitable Trust (G-1901-03687). The funding agencies have no role in data interpretation and publication of the results.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Type 1 diabetes (T1D) is an autoimmune disease, characterized by the presence of autoantibodies to protein and non-protein antigens. Here we report the identification of specific anti-carbohydrate antibodies (ACAs) that are associated with pathogenesis and progression to T1D. We compare circulatory levels of ACAs against 202 glycans in a cross-sectional cohort of T1D patients (n = 278) and healthy controls (n = 298), as well as in a longitudinal cohort (n = 112). We identify 11 clusters of ACAs associated with glycan function class. Clusters enriched for aminoglycosides, blood group A and B antigens, glycolipids, ganglio-series, and O-linked glycans are associated with progression to T1D. ACAs against gentamicin and its related structures, G418 and sisomicin, are also associated with islet autoimmunity. ACAs improve discrimination of T1D status of individuals over a model with only clinical variables and are potential biomarkers for T1D.
AB - Type 1 diabetes (T1D) is an autoimmune disease, characterized by the presence of autoantibodies to protein and non-protein antigens. Here we report the identification of specific anti-carbohydrate antibodies (ACAs) that are associated with pathogenesis and progression to T1D. We compare circulatory levels of ACAs against 202 glycans in a cross-sectional cohort of T1D patients (n = 278) and healthy controls (n = 298), as well as in a longitudinal cohort (n = 112). We identify 11 clusters of ACAs associated with glycan function class. Clusters enriched for aminoglycosides, blood group A and B antigens, glycolipids, ganglio-series, and O-linked glycans are associated with progression to T1D. ACAs against gentamicin and its related structures, G418 and sisomicin, are also associated with islet autoimmunity. ACAs improve discrimination of T1D status of individuals over a model with only clinical variables and are potential biomarkers for T1D.
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U2 - 10.1038/s41467-022-34341-2
DO - 10.1038/s41467-022-34341-2
M3 - Article
C2 - 36316364
AN - SCOPUS:85140936101
SN - 2041-1723
VL - 13
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 6527
ER -
