TY - CHAP
T1 - Use of tissue microarray to facilitate oncology research
AU - Gouveris, Panagiotis
AU - Weinberger, Paul M.
AU - Psyrri, Amanda
PY - 2010
Y1 - 2010
N2 - HPV-positive oropharyngeal squamous cell carcinomas (OSCC) represent a distinct disease entity from traditional OSCC. We hypothesized that for HPV DNA-positive cases, p16 expression status differentiates the biologically relevant ones. We determined HPV16DNA viral load in a cohort of 79 oropharyngeal squamous cell cancers by real-time polymerase chain reaction (PCR). We used cervical cancer as a disease model for HPV-initiated epithelial cancer. In cervical cancer, p53 and Rb expression is reduced, while p16 expression is increased. We used TMA technology to facilitate interrogation of this cohort for p53, Rb, and p16 protein expression using a quantitative, in situ method of protein analysis (AQUA analysis). Our results indeed delineate three biologically and clinically distinct types of oropharyngeal squamous cell cancers based on HPV-DNA determination and p16 expression status: one class of HPV-negative/p16-nonexpressing (HPV-negative), one class of HPV-positive/p16- nonexpressing (HPV-inactive), and one class of HPV positive/p16-expressing (HPV-active) oropharyngeal tumors. We demonstrated that only the HPV-active tumors share a similar molecular phenotype to cervical cancers, and are the ones associated with favorable prognosis.
AB - HPV-positive oropharyngeal squamous cell carcinomas (OSCC) represent a distinct disease entity from traditional OSCC. We hypothesized that for HPV DNA-positive cases, p16 expression status differentiates the biologically relevant ones. We determined HPV16DNA viral load in a cohort of 79 oropharyngeal squamous cell cancers by real-time polymerase chain reaction (PCR). We used cervical cancer as a disease model for HPV-initiated epithelial cancer. In cervical cancer, p53 and Rb expression is reduced, while p16 expression is increased. We used TMA technology to facilitate interrogation of this cohort for p53, Rb, and p16 protein expression using a quantitative, in situ method of protein analysis (AQUA analysis). Our results indeed delineate three biologically and clinically distinct types of oropharyngeal squamous cell cancers based on HPV-DNA determination and p16 expression status: one class of HPV-negative/p16-nonexpressing (HPV-negative), one class of HPV-positive/p16- nonexpressing (HPV-inactive), and one class of HPV positive/p16-expressing (HPV-active) oropharyngeal tumors. We demonstrated that only the HPV-active tumors share a similar molecular phenotype to cervical cancers, and are the ones associated with favorable prognosis.
KW - AQUA
KW - HPV16
KW - Oropharyngeal squamous cell cancer
KW - Quantitative immunohistochemistry
KW - Real-time polymerase chain reaction (PCR)
KW - p16
UR - http://www.scopus.com/inward/record.url?scp=77953274749&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953274749&partnerID=8YFLogxK
U2 - 10.1007/978-1-60761-663-4_15
DO - 10.1007/978-1-60761-663-4_15
M3 - Chapter
C2 - 20217582
AN - SCOPUS:77953274749
SN - 9781607616627
T3 - Methods in Molecular Biology
SP - 239
EP - 250
BT - Microarray Methods for Drug Discovery
A2 - Chittur, Sridar V.
ER -