TY - JOUR
T1 - Utility of targeted, amplicon-based deep sequencing to detect resistance to first-line tuberculosis drugs in Botswana
AU - Wang, Qiao
AU - Modongo, Chawangwa
AU - Allender, Christopher
AU - Engelthaler, David M.
AU - Warren, Robin M.
AU - Zetola, Nicola M.
AU - Shin, Sanghyuk S.
N1 - Publisher Copyright:
Copyright © 2019 American Society for Microbiology. All Rights Reserved.
PY - 2019
Y1 - 2019
N2 - Multidrug-resistant tuberculosis (TB) is an alarming threat, and targeted deep sequencing (DS) may be an effective method for rapid identification of drug-resistant profiles, including detection of heteroresistance. We evaluated the sensitivity and specificity of targeted DS versus phenotypic drug susceptibility testing (pDST) among patients starting first-line anti-TB therapy in Botswana. Overall, we found high concordance between DS and pDST. Lower sensitivity of DS, which targets established high-confidence resistance variants, was observed for detecting isoniazid resistance among HIV-infected patients.
AB - Multidrug-resistant tuberculosis (TB) is an alarming threat, and targeted deep sequencing (DS) may be an effective method for rapid identification of drug-resistant profiles, including detection of heteroresistance. We evaluated the sensitivity and specificity of targeted DS versus phenotypic drug susceptibility testing (pDST) among patients starting first-line anti-TB therapy in Botswana. Overall, we found high concordance between DS and pDST. Lower sensitivity of DS, which targets established high-confidence resistance variants, was observed for detecting isoniazid resistance among HIV-infected patients.
KW - Diagnostics
KW - Drug-resistant tuberculosis
KW - HIV infections
KW - Heteroresistance
KW - Next-generation sequencing
KW - Single-molecule overlapping reads
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U2 - 10.1128/AAC.00982-19
DO - 10.1128/AAC.00982-19
M3 - Article
C2 - 31405858
AN - SCOPUS:85073764550
SN - 0066-4804
VL - 63
JO - Antimicrobial agents and chemotherapy
JF - Antimicrobial agents and chemotherapy
IS - 11
M1 - e0098219
ER -