Utility of targeted, amplicon-based deep sequencing to detect resistance to first-line tuberculosis drugs in Botswana

Qiao Wang; Chawangwa Modongo; Christopher Allender; David M. Engelthaler; Robin M. Warren; Nicola M. Zetola; Sanghyuk S. Shin

doi:10.1128/AAC.00982-19

Utility of targeted, amplicon-based deep sequencing to detect resistance to first-line tuberculosis drugs in Botswana

Qiao Wang, Chawangwa Modongo, Christopher Allender, David M. Engelthaler, Robin M. Warren, Nicola M. Zetola, Sanghyuk S. Shin

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Multidrug-resistant tuberculosis (TB) is an alarming threat, and targeted deep sequencing (DS) may be an effective method for rapid identification of drug-resistant profiles, including detection of heteroresistance. We evaluated the sensitivity and specificity of targeted DS versus phenotypic drug susceptibility testing (pDST) among patients starting first-line anti-TB therapy in Botswana. Overall, we found high concordance between DS and pDST. Lower sensitivity of DS, which targets established high-confidence resistance variants, was observed for detecting isoniazid resistance among HIV-infected patients.

Original language	English (US)
Article number	e0098219
Journal	Antimicrobial agents and chemotherapy
Volume	63
Issue number	11
DOIs	https://doi.org/10.1128/AAC.00982-19
State	Published - 2019
Externally published	Yes

Keywords

Diagnostics
Drug-resistant tuberculosis
HIV infections
Heteroresistance
Next-generation sequencing
Single-molecule overlapping reads

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/AAC.00982-19

Cite this

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title = "Utility of targeted, amplicon-based deep sequencing to detect resistance to first-line tuberculosis drugs in Botswana",

abstract = "Multidrug-resistant tuberculosis (TB) is an alarming threat, and targeted deep sequencing (DS) may be an effective method for rapid identification of drug-resistant profiles, including detection of heteroresistance. We evaluated the sensitivity and specificity of targeted DS versus phenotypic drug susceptibility testing (pDST) among patients starting first-line anti-TB therapy in Botswana. Overall, we found high concordance between DS and pDST. Lower sensitivity of DS, which targets established high-confidence resistance variants, was observed for detecting isoniazid resistance among HIV-infected patients.",

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language = "English (US)",

volume = "63",

journal = "Antimicrobial agents and chemotherapy",

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T1 - Utility of targeted, amplicon-based deep sequencing to detect resistance to first-line tuberculosis drugs in Botswana

AU - Wang, Qiao

AU - Modongo, Chawangwa

AU - Allender, Christopher

AU - Engelthaler, David M.

AU - Warren, Robin M.

AU - Zetola, Nicola M.

AU - Shin, Sanghyuk S.

PY - 2019

Y1 - 2019

N2 - Multidrug-resistant tuberculosis (TB) is an alarming threat, and targeted deep sequencing (DS) may be an effective method for rapid identification of drug-resistant profiles, including detection of heteroresistance. We evaluated the sensitivity and specificity of targeted DS versus phenotypic drug susceptibility testing (pDST) among patients starting first-line anti-TB therapy in Botswana. Overall, we found high concordance between DS and pDST. Lower sensitivity of DS, which targets established high-confidence resistance variants, was observed for detecting isoniazid resistance among HIV-infected patients.

AB - Multidrug-resistant tuberculosis (TB) is an alarming threat, and targeted deep sequencing (DS) may be an effective method for rapid identification of drug-resistant profiles, including detection of heteroresistance. We evaluated the sensitivity and specificity of targeted DS versus phenotypic drug susceptibility testing (pDST) among patients starting first-line anti-TB therapy in Botswana. Overall, we found high concordance between DS and pDST. Lower sensitivity of DS, which targets established high-confidence resistance variants, was observed for detecting isoniazid resistance among HIV-infected patients.

KW - Diagnostics

KW - Drug-resistant tuberculosis

KW - HIV infections

KW - Heteroresistance

KW - Next-generation sequencing

KW - Single-molecule overlapping reads

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Utility of targeted, amplicon-based deep sequencing to detect resistance to first-line tuberculosis drugs in Botswana

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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