TY - JOUR
T1 - Utility of targeted, amplicon-based deep sequencing to detect resistance to first-line tuberculosis drugs in Botswana
AU - Wang, Qiao
AU - Modongo, Chawangwa
AU - Allender, Christopher
AU - Engelthaler, David M.
AU - Warren, Robin M.
AU - Zetola, Nicola M.
AU - Shin, Sanghyuk S.
N1 - Funding Information:
This study was funded by the National Institutes of Health (grants R01AI097045, P30AI45008, K01AI118559, and R21AI120838). The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Publisher Copyright:
Copyright © 2019 American Society for Microbiology. All Rights Reserved.
PY - 2019
Y1 - 2019
N2 - Multidrug-resistant tuberculosis (TB) is an alarming threat, and targeted deep sequencing (DS) may be an effective method for rapid identification of drug-resistant profiles, including detection of heteroresistance. We evaluated the sensitivity and specificity of targeted DS versus phenotypic drug susceptibility testing (pDST) among patients starting first-line anti-TB therapy in Botswana. Overall, we found high concordance between DS and pDST. Lower sensitivity of DS, which targets established high-confidence resistance variants, was observed for detecting isoniazid resistance among HIV-infected patients.
AB - Multidrug-resistant tuberculosis (TB) is an alarming threat, and targeted deep sequencing (DS) may be an effective method for rapid identification of drug-resistant profiles, including detection of heteroresistance. We evaluated the sensitivity and specificity of targeted DS versus phenotypic drug susceptibility testing (pDST) among patients starting first-line anti-TB therapy in Botswana. Overall, we found high concordance between DS and pDST. Lower sensitivity of DS, which targets established high-confidence resistance variants, was observed for detecting isoniazid resistance among HIV-infected patients.
KW - Diagnostics
KW - Drug-resistant tuberculosis
KW - HIV infections
KW - Heteroresistance
KW - Next-generation sequencing
KW - Single-molecule overlapping reads
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U2 - 10.1128/AAC.00982-19
DO - 10.1128/AAC.00982-19
M3 - Article
C2 - 31405858
AN - SCOPUS:85073764550
SN - 0066-4804
VL - 63
JO - Antimicrobial agents and chemotherapy
JF - Antimicrobial agents and chemotherapy
IS - 11
M1 - e0098219
ER -
