TY - JOUR
T1 - Wound healing properties of modified silver nanoparticles and their distribution in mouse organs after topical application
AU - Keleştemur, Seda
AU - Kilic, Ertugrul
AU - Uslu, Ünal
AU - Cumbul, Alev
AU - Ugur, Milas
AU - Akman, Süleyman
AU - Culha, Mustafa
PY - 2012
Y1 - 2012
N2 - Citrate reduced colloidal silver nanoparticles (c-AgNPs) as synthesized and modified with oligonucleotides (Oligo-AgNPs) are comparatively evaluated for their wound healing properties on animal models. The healing progress was monitored daily during nine days by measuring the wound diameter. The tissue samples from the healed regions were analyzed for epithelial damage, congestion, inflammatory cellinfiltration, fibroblast proliferation, and new collagen synthesis. The c-AgNPs and Oligo-AgNPs had statically significant impact on the healing process compared to control. The histological analysis revealed that the c-AgNPs and Oligo-AgNPs improved the congestion, inflammatory cellinfiltration, fibroblast proliferation and new collagen synthesis as compared to control. Although the fibroblast proliferation seems to be the same for both c-AgNPs and Oligo-AgNPs, the collagen synthesis is markedly improved with the Oligo-AgNPs. The atomic spectroscopy analysis of the samples from different tissues showed that the AgNPs applied topically to the skin does not pass through the other rgans. Our data suggest that topical application of Oligos-AgNPs improve wound healing by promoting increased collagen synthesis and tissue re-modeling without any side effects.
AB - Citrate reduced colloidal silver nanoparticles (c-AgNPs) as synthesized and modified with oligonucleotides (Oligo-AgNPs) are comparatively evaluated for their wound healing properties on animal models. The healing progress was monitored daily during nine days by measuring the wound diameter. The tissue samples from the healed regions were analyzed for epithelial damage, congestion, inflammatory cellinfiltration, fibroblast proliferation, and new collagen synthesis. The c-AgNPs and Oligo-AgNPs had statically significant impact on the healing process compared to control. The histological analysis revealed that the c-AgNPs and Oligo-AgNPs improved the congestion, inflammatory cellinfiltration, fibroblast proliferation and new collagen synthesis as compared to control. Although the fibroblast proliferation seems to be the same for both c-AgNPs and Oligo-AgNPs, the collagen synthesis is markedly improved with the Oligo-AgNPs. The atomic spectroscopy analysis of the samples from different tissues showed that the AgNPs applied topically to the skin does not pass through the other rgans. Our data suggest that topical application of Oligos-AgNPs improve wound healing by promoting increased collagen synthesis and tissue re-modeling without any side effects.
KW - Collagen synthesis inflammation
KW - Congestion
KW - Silver nanoparticles
KW - Wound healing
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U2 - 10.5101/nbe.v4i4.p170-176
DO - 10.5101/nbe.v4i4.p170-176
M3 - Article
AN - SCOPUS:84871745786
SN - 2150-5578
VL - 4
SP - 170
EP - 176
JO - Nano Biomedicine and Engineering
JF - Nano Biomedicine and Engineering
IS - 4
ER -