Characterization of Differentially Expressed miRNAs by CXCL12/SDF-1 in Human Bone Marrow Stromal Cells

Matthew L. Potter; Kathryn Smith; Sagar Vyavahare; Sandeep Kumar; Sudharsan Periyasamy-Thandavan; Mark Hamrick; Carlos M. Isales; William D. Hill; Sadanand Fulzele

doi:10.1515/bmc-2021-0015

Characterization of Differentially Expressed miRNAs by CXCL12/SDF-1 in Human Bone Marrow Stromal Cells

Matthew L. Potter, Kathryn Smith, Sagar Vyavahare, Sandeep Kumar, Sudharsan Periyasamy-Thandavan, Mark Hamrick, Carlos M. Isales, William D. Hill, Sadanand Fulzele

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Stromal cell-derived factor 1 (SDF-1) is known to influence bone marrow stromal cell (BMSC) migration, osteogenic differentiation, and fracture healing. We hypothesize that SDF-1 mediates some of its effects on BMSCs through epigenetic regulation, specifically via microRNAs (miRNAs). MiRNAs are small non-coding RNAs that target specific mRNA and prevent their translation. We performed global miRNA analysis and determined several miRNAs were differentially expressed in response to SDF-1 treatment. Gene Expression Omnibus (GEO) dataset analysis showed that these miRNAs play an important role in osteogenic differentiation and fracture healing. KEGG and GO analysis indicated that SDF-1 dependent miRNAs changes affect multiple cellular pathways, including fatty acid biosynthesis, thyroid hormone signaling, and mucin-type O-glycan biosynthesis pathways. Furthermore, bioinformatics analysis showed several miRNAs target genes related to stem cell migration and differentiation. This study's findings indicated that SDF-1 induces some of its effects on BMSCs function through miRNA regulation.

Original language	English (US)
Pages (from-to)	132-143
Number of pages	12
Journal	Biomolecular Concepts
Volume	12
Issue number	1
DOIs	https://doi.org/10.1515/bmc-2021-0015
State	Published - Jan 1 2021

Keywords

SDF-1
bone marrow stromal cells
miRNA

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Cellular and Molecular Neuroscience

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10.1515/bmc-2021-0015

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title = "Characterization of Differentially Expressed miRNAs by CXCL12/SDF-1 in Human Bone Marrow Stromal Cells",

abstract = "Stromal cell-derived factor 1 (SDF-1) is known to influence bone marrow stromal cell (BMSC) migration, osteogenic differentiation, and fracture healing. We hypothesize that SDF-1 mediates some of its effects on BMSCs through epigenetic regulation, specifically via microRNAs (miRNAs). MiRNAs are small non-coding RNAs that target specific mRNA and prevent their translation. We performed global miRNA analysis and determined several miRNAs were differentially expressed in response to SDF-1 treatment. Gene Expression Omnibus (GEO) dataset analysis showed that these miRNAs play an important role in osteogenic differentiation and fracture healing. KEGG and GO analysis indicated that SDF-1 dependent miRNAs changes affect multiple cellular pathways, including fatty acid biosynthesis, thyroid hormone signaling, and mucin-type O-glycan biosynthesis pathways. Furthermore, bioinformatics analysis showed several miRNAs target genes related to stem cell migration and differentiation. This study's findings indicated that SDF-1 induces some of its effects on BMSCs function through miRNA regulation.",

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author = "Potter, {Matthew L.} and Kathryn Smith and Sagar Vyavahare and Sandeep Kumar and Sudharsan Periyasamy-Thandavan and Mark Hamrick and Isales, {Carlos M.} and Hill, {William D.} and Sadanand Fulzele",

note = "Funding Information: This publication is based upon work supported in part by the National Institutes of Health AG036675 (National Institute on Aging-AG036675 S.F, W.D.H, M.H, C.S,). The funding mentioned above did not lead to any conflict of interest regarding the publication of this manuscript. Publisher Copyright: {\textcopyright} 2021 Matthew L. Potter et al., published by De Gruyter.",

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AU - Potter, Matthew L.

AU - Smith, Kathryn

AU - Vyavahare, Sagar

AU - Kumar, Sandeep

AU - Periyasamy-Thandavan, Sudharsan

AU - Hamrick, Mark

AU - Isales, Carlos M.

AU - Hill, William D.

AU - Fulzele, Sadanand

N1 - Funding Information: This publication is based upon work supported in part by the National Institutes of Health AG036675 (National Institute on Aging-AG036675 S.F, W.D.H, M.H, C.S,). The funding mentioned above did not lead to any conflict of interest regarding the publication of this manuscript. Publisher Copyright: © 2021 Matthew L. Potter et al., published by De Gruyter.

PY - 2021/1/1

Y1 - 2021/1/1

N2 - Stromal cell-derived factor 1 (SDF-1) is known to influence bone marrow stromal cell (BMSC) migration, osteogenic differentiation, and fracture healing. We hypothesize that SDF-1 mediates some of its effects on BMSCs through epigenetic regulation, specifically via microRNAs (miRNAs). MiRNAs are small non-coding RNAs that target specific mRNA and prevent their translation. We performed global miRNA analysis and determined several miRNAs were differentially expressed in response to SDF-1 treatment. Gene Expression Omnibus (GEO) dataset analysis showed that these miRNAs play an important role in osteogenic differentiation and fracture healing. KEGG and GO analysis indicated that SDF-1 dependent miRNAs changes affect multiple cellular pathways, including fatty acid biosynthesis, thyroid hormone signaling, and mucin-type O-glycan biosynthesis pathways. Furthermore, bioinformatics analysis showed several miRNAs target genes related to stem cell migration and differentiation. This study's findings indicated that SDF-1 induces some of its effects on BMSCs function through miRNA regulation.

AB - Stromal cell-derived factor 1 (SDF-1) is known to influence bone marrow stromal cell (BMSC) migration, osteogenic differentiation, and fracture healing. We hypothesize that SDF-1 mediates some of its effects on BMSCs through epigenetic regulation, specifically via microRNAs (miRNAs). MiRNAs are small non-coding RNAs that target specific mRNA and prevent their translation. We performed global miRNA analysis and determined several miRNAs were differentially expressed in response to SDF-1 treatment. Gene Expression Omnibus (GEO) dataset analysis showed that these miRNAs play an important role in osteogenic differentiation and fracture healing. KEGG and GO analysis indicated that SDF-1 dependent miRNAs changes affect multiple cellular pathways, including fatty acid biosynthesis, thyroid hormone signaling, and mucin-type O-glycan biosynthesis pathways. Furthermore, bioinformatics analysis showed several miRNAs target genes related to stem cell migration and differentiation. This study's findings indicated that SDF-1 induces some of its effects on BMSCs function through miRNA regulation.

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Characterization of Differentially Expressed miRNAs by CXCL12/SDF-1 in Human Bone Marrow Stromal Cells

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